For the first time, scientists have completed the sequencing of the stevia plant genome. Lead scientists from PureCircle Stevia Institute and KeyGene have unveiled this major breakthrough in research showing the annotated genome sequences of three cultivars of the plant. It is hoped this may provide a better understanding of key enzyme groups used by the plant to produce the steviol glycosides giving the sugar substitute its characteristic sweet taste. To enable acceleration of the traditional breeding of the plant, researchers identified several million potentially new markers in the assembled genomes. PureCircle’s non-GMO agriculture programme is strengthened by these findings. The research may help facilitate optimisation of the levels of steviol glycosides, including improvements in the levels of the well-known minor glycosides, Reb D and Reb M. The data has been integrated into CropPedia, a comprehensive bioinformatics platform developed by KeyGene for visualisation and analytics of all available genomic, transcriptomic and metabolomic stevia datasets. CropPedia enables chemists, biochemists, geneticists and agronomists to better understand the steviol glycoside biosynthesis pathways, and to rapidly create improved varieties using traditional breeding practices. Stevia is a plant-based, zero-calorie sweetener which has been approved by all major regulatory authorities globally for use in foods and beverages in over 150 countries. Ingredients developed as a result of this research could enable further reductions in sugar and calorie content of foods and beverages. This could benefit consumers around the world by helping them meet dietary guidelines calling for reductions in sugar and calorie intake for improved health.